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Engineering human neural tissue by 3D bioprinting

Chapter


Abstract


  • © 2018, Springer Science+Business Media, LLC, part of Springer Nature. Bioprinting provides an opportunity to produce three-dimensional (3D) tissues for biomedical research and translational drug discovery, toxicology, and tissue replacement. Here we describe a method for fabricating human neural tissue by 3D printing human neural stem cells with a bioink, and subsequent gelation of the bioink for cell encapsulation, support, and differentiation to functional neurons and supporting neuroglia. The bioink uniquely comprises the polysaccharides alginate, water-soluble carboxymethyl-chitosan, and agarose. Importantly, the method could be adapted to fabricate neural and nonneural tissues from other cell types, with the potential to be applied for both research and clinical product development.

Publication Date


  • 2018

Citation


  • Gu, Q., Tomaskovic-Crook, E., Wallace, G. & Crook, J. (2018). Engineering human neural tissue by 3D bioprinting. Biomaterials for tissue engineering: methods and protocols (pp. 129-138). United States: Humana Press, Inc..

International Standard Book Number (isbn) 13


  • 9781493977413

Scopus Eid


  • 2-s2.0-85045875880

Book Title


  • Biomaterials for tissue engineering: methods and protocols

Start Page


  • 129

End Page


  • 138

Place Of Publication


  • United States

Abstract


  • © 2018, Springer Science+Business Media, LLC, part of Springer Nature. Bioprinting provides an opportunity to produce three-dimensional (3D) tissues for biomedical research and translational drug discovery, toxicology, and tissue replacement. Here we describe a method for fabricating human neural tissue by 3D printing human neural stem cells with a bioink, and subsequent gelation of the bioink for cell encapsulation, support, and differentiation to functional neurons and supporting neuroglia. The bioink uniquely comprises the polysaccharides alginate, water-soluble carboxymethyl-chitosan, and agarose. Importantly, the method could be adapted to fabricate neural and nonneural tissues from other cell types, with the potential to be applied for both research and clinical product development.

Publication Date


  • 2018

Citation


  • Gu, Q., Tomaskovic-Crook, E., Wallace, G. & Crook, J. (2018). Engineering human neural tissue by 3D bioprinting. Biomaterials for tissue engineering: methods and protocols (pp. 129-138). United States: Humana Press, Inc..

International Standard Book Number (isbn) 13


  • 9781493977413

Scopus Eid


  • 2-s2.0-85045875880

Book Title


  • Biomaterials for tissue engineering: methods and protocols

Start Page


  • 129

End Page


  • 138

Place Of Publication


  • United States