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Clinically amendable, defined, and rapid induction of human brain organoids from induced pluripotent stem cells

Journal Article


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Abstract


  • Human brain organoids provide opportunities to produce three-dimensional (3D) brain-like tissues for

    biomedical research and translational drug discovery, toxicology, and tissue replacement. Here we describe

    a protocol for rapid and defined induction of brain organoids from human induced pluripotent stem cells

    (iPSCs), using commercially available culture and differentiation media and a cheap, easy to handle and

    clinically approved semisynthetic hydrogel. Importantly, the methodology is uncomplicated, well-defined,

    and reliable for reproducible and scalable organoid generation, and amendable to principles of current good

    laboratory practice (cGLP), with the potential for prospective adaptation to current good manufacturing

    practice (cGMP) toward clinical compliance.

Publication Date


  • 2019

Citation


  • Tomaskovic-Crook, E. & Crook, J. M. (2019). Clinically amendable, defined, and rapid induction of human brain organoids from induced pluripotent stem cells. Organoids, 1576 13-22.

Scopus Eid


  • 2-s2.0-85053019244

Ro Full-text Url


  • https://ro.uow.edu.au/cgi/viewcontent.cgi?article=4947&context=aiimpapers

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/3892

Has Global Citation Frequency


Number Of Pages


  • 9

Start Page


  • 13

End Page


  • 22

Volume


  • 1576

Place Of Publication


  • United Kingdom

Abstract


  • Human brain organoids provide opportunities to produce three-dimensional (3D) brain-like tissues for

    biomedical research and translational drug discovery, toxicology, and tissue replacement. Here we describe

    a protocol for rapid and defined induction of brain organoids from human induced pluripotent stem cells

    (iPSCs), using commercially available culture and differentiation media and a cheap, easy to handle and

    clinically approved semisynthetic hydrogel. Importantly, the methodology is uncomplicated, well-defined,

    and reliable for reproducible and scalable organoid generation, and amendable to principles of current good

    laboratory practice (cGLP), with the potential for prospective adaptation to current good manufacturing

    practice (cGMP) toward clinical compliance.

Publication Date


  • 2019

Citation


  • Tomaskovic-Crook, E. & Crook, J. M. (2019). Clinically amendable, defined, and rapid induction of human brain organoids from induced pluripotent stem cells. Organoids, 1576 13-22.

Scopus Eid


  • 2-s2.0-85053019244

Ro Full-text Url


  • https://ro.uow.edu.au/cgi/viewcontent.cgi?article=4947&context=aiimpapers

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/3892

Has Global Citation Frequency


Number Of Pages


  • 9

Start Page


  • 13

End Page


  • 22

Volume


  • 1576

Place Of Publication


  • United Kingdom