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3D Bioprinting Electrically Conductive Bioink with Human Neural Stem Cells for Human Neural Tissues

Journal Article


Abstract


  • Bioprinting cells with an electrically conductive bioink provides an opportunity to produce three-dimensional (3D) cell-laden constructs with the option of electrically stimulating cells in situ during and after tissue development. We and others have demonstrated the use of electrical stimulation (ES) to influence cell behavior and function for a more biomimetic approach to tissue engineering. Here, we detail a previously published method for 3D printing an electrically conductive bioink with human neural stem cells (hNSCs) that are subsequently differentiated. The differentiated tissue constructs comprise functional neurons and supporting neuroglia and are amenable to ES for the purposeful modulation of neural activity. Importantly, the method could be adapted to fabricate and stimulate neural and nonneural tissues from other cell types, with the potential to be applied for both research- and clinical-product development.

Publication Date


  • 2020

Citation


  • Tomaskovic-Crook, E. & Crook, J. (2020). 3D Bioprinting Electrically Conductive Bioink with Human Neural Stem Cells for Human Neural Tissues. Methods in Molecular Biology, 2140 159-170.

Scopus Eid


  • 2-s2.0-85082292818

Number Of Pages


  • 11

Start Page


  • 159

End Page


  • 170

Volume


  • 2140

Place Of Publication


  • United States

Abstract


  • Bioprinting cells with an electrically conductive bioink provides an opportunity to produce three-dimensional (3D) cell-laden constructs with the option of electrically stimulating cells in situ during and after tissue development. We and others have demonstrated the use of electrical stimulation (ES) to influence cell behavior and function for a more biomimetic approach to tissue engineering. Here, we detail a previously published method for 3D printing an electrically conductive bioink with human neural stem cells (hNSCs) that are subsequently differentiated. The differentiated tissue constructs comprise functional neurons and supporting neuroglia and are amenable to ES for the purposeful modulation of neural activity. Importantly, the method could be adapted to fabricate and stimulate neural and nonneural tissues from other cell types, with the potential to be applied for both research- and clinical-product development.

Publication Date


  • 2020

Citation


  • Tomaskovic-Crook, E. & Crook, J. (2020). 3D Bioprinting Electrically Conductive Bioink with Human Neural Stem Cells for Human Neural Tissues. Methods in Molecular Biology, 2140 159-170.

Scopus Eid


  • 2-s2.0-85082292818

Number Of Pages


  • 11

Start Page


  • 159

End Page


  • 170

Volume


  • 2140

Place Of Publication


  • United States