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Bioprinting 3D Human Induced Pluripotent Stem Cell Constructs for Multilineage Tissue Engineering and Modeling

Journal Article


Abstract


  • Bioprinting human pluripotent stem cells (PSCs) provides an opportunity to produce three-dimensional (3D) cell-laden constructs with the potential to be differentiated in vitro to all tissue types of the human body. Here, we detail a previously published method for 3D printing human induced pluripotent stem cells (iPSCs; also applicable to human embryonic stem cells) within a clinically amenable bioink (also described in Chapter 10) that is cross-linked to a 3D construct. The printed iPSCs continue to have self-replicating and multilineage cell induction potential in situ, and the constructs are robust and amenable to different differentiation protocols for fabricating diverse tissue types, with the potential to be applied for both research- and clinical-product development.

Publication Date


  • 2020

Citation


  • Crook, J. M., & Tomaskovic-Crook, E. (2020). Bioprinting 3D Human Induced Pluripotent Stem Cell Constructs for Multilineage Tissue Engineering and Modeling. Unknown Journal, 2140, 251-258. doi:10.1007/978-1-0716-0520-2_17

Scopus Eid


  • 2-s2.0-85082381010

Web Of Science Accession Number


Start Page


  • 251

End Page


  • 258

Volume


  • 2140

Abstract


  • Bioprinting human pluripotent stem cells (PSCs) provides an opportunity to produce three-dimensional (3D) cell-laden constructs with the potential to be differentiated in vitro to all tissue types of the human body. Here, we detail a previously published method for 3D printing human induced pluripotent stem cells (iPSCs; also applicable to human embryonic stem cells) within a clinically amenable bioink (also described in Chapter 10) that is cross-linked to a 3D construct. The printed iPSCs continue to have self-replicating and multilineage cell induction potential in situ, and the constructs are robust and amenable to different differentiation protocols for fabricating diverse tissue types, with the potential to be applied for both research- and clinical-product development.

Publication Date


  • 2020

Citation


  • Crook, J. M., & Tomaskovic-Crook, E. (2020). Bioprinting 3D Human Induced Pluripotent Stem Cell Constructs for Multilineage Tissue Engineering and Modeling. Unknown Journal, 2140, 251-258. doi:10.1007/978-1-0716-0520-2_17

Scopus Eid


  • 2-s2.0-85082381010

Web Of Science Accession Number


Start Page


  • 251

End Page


  • 258

Volume


  • 2140